By B. J. Richardson
Inherited enzyme diversifications, studied utilizing electrophoresis, can be utilized as markers for the identity of people, inhabitants constitution research, the delineation of species barriers and phytogenetic reconstruction. the aim of this e-book is to explain, in one handy guide, all of the theoretical and sensible issues suitable to these desiring to use electrophoresis as a device for answering such questions.
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Extra info for Allozyme Electrophoresis. A Handbook for Animal Systematics and Population Studies
ALTERNATIVE POPULATION MODELS A population consists of individuals scattered, at various densities, throughout the range of the species. These individuals are not independent but interact in various ways with the environment and with one another. Most importantly, interactions between individuals produce the next gener ation, which is, as a result, the product of historical events occurring before as well as after its initiation. Some of these events affect the genetic structure of the population, and such facts can be used to develop or test ideas about the structure of a population, notably the relationship between groups of animals in different parts of the range of the species.
In the same way that allozyme electrophoresis can be valuable for the study of allopatric populations, it can be used to elucidate complex situations involving parthenogenic forms and hybrid zones. In the case of parthenogenic forms, the decision to be made is whether the level of genetic divergence is sufficient (by analogy with related sexually reproducing species) to restrict interbreeding if the forms were able to do so. Hybrid zones result when interbreeding occurs in a more or less limited region between two forms which continue to maintain their genetic distinctiveness outside the zone of hybridization.
DNA differences) at a protein locus are detectable using allozyme electrophoresis (Kreitman, 1983). Possible sources of such allelic variation at the DNA level are: A. Allelic variation in introns The segments of the DNA present as introns are never translated to form part of the protein under study and therefore allelic variation in introns is not detectable at the level of allozyme electrophoresis. B. e. the genetic code is redundant. Usually the various triplets differ by only one base, commonly in the third position.
Allozyme Electrophoresis. A Handbook for Animal Systematics and Population Studies by B. J. Richardson